A quantitative polymerase chain reaction (qPCR) leverages the power of the traditional polymerase chain reaction (PCR) to determine how much target DNA is present by measuring fluorescent signal associated with DNA amplification. We have experience developing assays with both SYBR Green and sequence-specific probes to fit a range of budget and timeline needs.
SYBR Green is an intercalating dye which is incorporated into any double-stranded DNA during synthesis. These assays are less specific but much quicker to develop, requiring only the synthesis of primers. SYBR is a cost-effective reagent for clients looking to analyze a single target.
Probes contain a fluorophore bind to a specific sequence of single-stranded template DNA. As new DNA is synthesized, the probe is degraded and the fluorophore released. These assays are more specific and have the capability to detect up to three targets in the same tube with our instrument.