Our catalog of primary immune effector cells derive from whole blood obtained from anonymous donors. Individual subsets are affinity purified by negative selection when possible, or positive selection when necessary. The methods deployed are disclosed within the specification data so that you can decide if the process used might adversely impact the biology you wish to study. We deploy a variety of methodologies to evaluate subset functions. These include ELISA, Elispot and/or cell-based assays (including chemotaxis) for the detection of cytokine and chemokine activities, LDH release for cytolytic activity, and total CFSE and/or ATP content to assess proliferation. We also provide flow cytometric analyses to monitor temporal changes in protein expression.